3D Printed Engineered Nano-Composite Templates for Bone Regeneration – 3DPRENT

Recent clinical studies performed by our group and other clinical partners in Europe, propose alternatives to conventional treatment modalities by using the concept of tissue engineering in which biomaterials (scaffolds) are used to deliver mesenchymal stem cells (MSC) and/or growth factors. Although there have been some successes, bone tissue engineering needs to overcome several challenges to meet clinical and commercialization needs. Among these challenges, the limitations of scaffolding biomaterials that can resemble macro to nanoscale structures of native bone tissues.

In 3DPRENT, nanocellulose-based hydrogel will be functionalized with nano-hydroxyapatite for osteoconductivity and nanodiamond particles (nDP) to deliver vascular endothelial growth factor (VEGF) to bioprint mesenchymal stem cells (MSC). For mechanical stability and vascularization, hydrogel layers will be reinforced with 3D printed microchannel network of a thermoplastic polymer. 3DPRENT will develop not only outstanding basic scientific knowledge but also sustainable solutions and innovations based on nanotechnology, 3D printing and stem cells thus improving health and promoting new medical technology to meet the needs of society.

Publications & Conferences

Published Articles

Submitted Articles

Conferences

All Publications

EORS2023

ESB2023

Rashad, A., Grøndahl, M., Heggset, E. B., Mustafa, K., & Syverud, K. (2023). Responses of Rat Mesenchymal Stromal Cells to Nanocellulose with Different Functional Groups. ACS Applied Bio Materials, 6(3), 987-998.

Shanbhag, S., Kampleitner, C., Al-Sharabi, N., Mohamed-Ahmed, S., Apaza Alccayhuaman, K. A., Heimel, P., … & Gruber, R. (2023). Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects.

Musthafa, HS., Walker, J., Rahman, T., Bjørkum, A., Mustafa, K., Velauthapillai, D., (2023). In-Silico Prediction of Mechanical Behaviour of Uniform Gyroid Scaffolds Affected by Its Design Parameters for Bone Tissue Engineering Applications. Computation, 11(9), 181,

Hara, K., Hellem, E., Yamada, S., Sariibrahimoglu, K., Mølster, A., Gjerdet, N. R., … & Yassin, M. A. (2022). Efficacy of treating segmental bone defects through endochondral ossification: 3D printed designs and bone metabolic activities. Materials Today Bio, 14, 100237

Schweinitzer, S., Kadousaraei, MJ., Aydin, MS. Rashad, A., (2023). Measuring cell proliferation in bioprinting research, Biomedical Materials.

Korkeamäki, JT, Rashad, A., (2023). Biomimetic Highly Porous Nanocellulose-Nanohydroxyapatite Scaffolds for Bone Tissue Engineering, Cellulose

Montanaria, M., Korkeamäki, JT., Campodonia, E., Mustafa, K., Sandria M.,Rashad, A., (2022). Bioprinting of biomimetic nanostructured hydroxyapatites within HEV-light curable hydrogel for bone tissue engineering.

Perfusable tissue bioprinted into a 3D-printed tailored bioreactor system” by Marius Gensler et al. at

Oral Presentations

Aydin, MS., Luciani, T., Mohamed-Ahmed, S., Yassin, MA., Mustafa, K., Rashad, A., (2023), 3D printing of PCL to fabricate porous scaffolds for bone tissue engineering applications, 31st Annual Meeting of the European Orthopedic Research Society (Oral Presentation).

Aydin, MS., Luciani, T., Mohamed-Ahmed, S., Mustafa, K., Rashad, A. (2023), Combining phase separation and particulate leaching with 3D printing to fabricate porous scaffolds for bone tissue engineering applications. 31st Annual Meeting of the European Orthopedic Research Society (Oral Presentation).

Aydin, MS., Luciani, T., Mohamed-Ahmed, S., Yassin, MA., Mustafa, K., Rashad, A. (2023), 3D Printed Polymeric Scaffolds with Enhanced Porosity for Bone Tissue Engineering, Mohn Research Centre 1st International Meeting on Regenerative Medicine for Regenerative Medicine (Oral Presentation).

Johansen Å., Korkeamäki JK., Miettinen, S., Mustafa K., (2023). Vascularization under optimized co-culture designs., MRCRM 1st International Meeting on Regenerative Medicine 2023 (Oral presentation)

Nicolae, CV., Serafim, A., Stavarache, CE., Ginghină, RE., Steinmüller-Nethl, D., Lackner, J., Syverud, K., Sandri, M., Mustafa, K., Rashad, A., Stancu, IC, (2023). Development of bioinspired 3D printable nanostructured formulations for bone tissue regeneration, 3^rd International Conference on Bioengineering and Polymer Science, Bucharest, Romania, (Oral Presentation).

Syverud, K., Rashad, A., Mustafa, K., (2023), Nanocellulose in tissue engineering – effects of surface chemistry. 5^th International cellulose conference, Hiroshima, Japan, (Oral Presentation).

Velu, AJ., Thirunavukkarasu, P., Rahman, T., Velauthapillai, D., (2023). In silico study on biodegradation and crystallization of polymeric implants, Advanced Materials for Clean Energy and Health Applications, India, (Oral Presentation).

Velu, AJ., Thirunavukkarasu, P., Musthafa, HS., Velauthapillai, D., (2022). Degradation behavior of biodegradable polymers as a medical implants, Technological Advances in Science Medicine and Engineering, Online, (Oral Presentation).

Velu, AJ., Thirunavukkarasu, P., Velauthapillai, D., (2021). Computational modeling and simulation study on degradation of scaffolds in bone tissue engineering, Technological Advances in Science Medicine and Engineering, Online, (Oral Presentation).

Poster Presentations

Aydin, MS., Luciani, T., Mohamed-Ahmed, S., Mustafa, K., Rashad, A. (2023). Combining phase separation and particulate leaching with 3D printing to fabricate porous scaffolds for bone tissue engineering applications. 33rd Annual Conference of the European Society for Biomaterials, Davos, Switzerland (Poster).

Kadousaraei, MJ., Yamada S., Wiik MEF., Mohamed-Ahmed, S., Rashad, A., Mustafa, K., (2023). Effect of photocuring parameters on viability and activity of mesenchymal stem cells, 33rd Annual Conference of the European Society for Biomaterials, Davos, Switzerland, (Poster).

Luciani, T., Aydin, MS., Mohamed-Ahmed, S., Mustafa, K., Rashad, A. (2023), Polycaprolactone 3D printed scaffolds with internal porosity towards clinical applications of bone regeneration. 33^rd Annual Conference of the European Society for Biomaterials, Davos, Switzerland (Poster).

Kadousaraei, MJ., Yamada S., Wiik MEF., Mohamed-Ahmed, S., Rashad, A., Syverud K., Mustafa, K., (2022). Cellulose Nanofibrils Improve the Printability of Gelatin Ink for Tissue Engineering Applications, Recent advances on cellulose nanotechnology research, Trondheim, Norway, (Poster).

Kadousaraei, MJ., Jamshidi-Adegani, F., Vakilian, S., Osman T., Rashad, A., Mustafa, K., (2022). Successful 3D-Bioprinting of Human Bone Marrow Mesenchymal Stem Cells for Bone Tissue Engineering Applications, Nordic Young Scientists in Oral Research Conference (NYSCO), Aarhus University, Aarhus, Denmark, (Poster).

Kadousaraei, MJ., Schweinitzer, S., Luciani T., Rashad, A., Mustafa, K., (2022). Response of Stem Cells to 3D bioprinted Alginate-Gelatin-based Structures, NorDoc PhD Conference, University of Bergen, Bergen, Norway, (Poster)

Aydin, MS., Luciani, T., Mohamed-Ahmed, S., Yassin, MA., Mustafa, K., Rashad, (2022). Phase separation and particulate leaching integrated 3D printing of porous scaffolds for bone tissue engineering applications. NorDoc PhD Conference, University of Bergen, Bergen, Norway (Poster).

Luciani, T., Kadousaraei, MJ., Torelli, F., Solheim, MC., Mohamed-Ahmed, S., Mustafa, K., Rashad, A., (2022). Synergistic effect of surface etching and inner design of 3D printed scaffolds for bone tissue engineering. NorDoc PhD Conference, University of Bergen, Bergen, Norway (Poster).

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