Recent project activity
Increased emphasis is being placed on translational research discoveries, which will bring the use of stem cells in laboratory studies into the clinical setting. However, only few early stage clinical studies with a limited number of patients have proven safety of this novel regenerative treatment. According to the clinical database (www.clinicaltrials.gov) and Medline, it appears that our research is at the forefront of regenerative medicine as we have successfully performed an early stage pilot clinical study using stem cell therapy in 11 patients for reconstruction of alveolar bone with excellent results at the 24 month examination. These results pave the way for improved and well-designed phase II clinical trial using stem cell therapy for bone regeneration.
The StemRegBone project consists of a number of sub-projects including therapeutic and safety assessment in clinical trial and optimization of MSC expansion in preclinical studies.
Reconstruction of jaw/alveolar bones using stem cells and biomaterials.
Atrophied bone has a limited spontaneous healing capacity. A clinical trial is designed to demonstrate the safety and efficacy of mesenchymal stem cells (MSC) for making new bone.
Monitoring grafted MSC using a minimally invasive diagnostic concept
The interactions between the transplanted MSCs and host immune cells is under elucidation by liquid biopsy to assess safety and immune efficacy of autologous bone marrow MSC therapy
Optimization and production of clinical grade MSC
For the production of clinical grade, xeno-free culture condition is to be adopted. In this project, the efficacy of using Human Platelet Lysates (HPLs) as an alternative to animal serum (i.e., fatal bovine serum) is tested for MSC expansion for the application of cell transplantation therapy.
Optimization of capacity and cost of ex-vivo expansion using bioreactors to facilitate commercialization of MSC as ATMP
In bone tissue engineering, various bioreactor systems are used to control MSC fate mechanically. In the project, mechanical regulation of MSC dynamics is assessed by applying dynamic culture conditions.
Publication 2019-2021
- Yamada S et al.Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor.Journal of Tissue Engineering(2021)
- Yamada S et al.Surface activation with oxygen plasma promotes osteogenesis with enhanced extracellular matrix formation in three-dimensional microporous scaffolds.Journal of Biomedical Materials Research Part A(2021)
- Yamada S et al.Scaffolds in Periodontal Regenerative Treatment.Dental Clinics of North America(2021)
- Shanbhag S et al.Spheroid coculture of human gingiva-derived progenitor cells with endothelial cells in modified platelet lysate hydrogels.Front Bioeng Biotechnol(2021)
- Shanbhag S et al.Bone regeneration in rat calvarial defects using suspension or spheroid mesenchymal stromal cells.Stem Cell ResTher.In press(2021)
- Helgeland E et al.3D printed gelatin scaffolds for temporomandibular joint cartilage regeneration. Biomed Phys Eng Express (2021)
- Helgeland E et al.Dual-crosslinked 3D printed gelatin scaffolds with potential for temporomandibular joint cartilage regeneration. Biomed Mater (2021)
- Jain S et al.Understanding of how the properties of medical grade lactide based copolymer scaffolds influence adipose tissue regeneration:Sterilization and a systematic in vitro assessment. Materials Science&Engineering C (2021)
- Mohamed-Ahmed I et al.Comparison of bone regenerative capacity of donor-matched human adipose-derived and bone marrow mesenchymal stem cells.Cell Tissue Res(2021)
- Gjerde C et al.Patient experience following iliac crest-derived alveolar bone grafting and implant placement.International J Implant Dentistry.(2020)
- Shanbhag S et al.Xeno-free spheroids of human gingiva-derived progenitor cells for bone tissue engineering.Front Bioeng Biotechnol.(2020)
- Shanbhag S et al.Influence of platelets storage time on human platelet lysates and platelet lysate-expanded mesenchymal stromal cells for bone tissue engineering.Stem Cell Res Ther.(2020)
- Helgeland E et al.Angiostatin-functionalized collagen scaffolds suppress angiogenesis but do not induce chondrogenesis by mesenchymal stromal cells in vivo.J Oral Sci.(2020)
- Jain S et al.Engineering 3D degradable,pliable scaffolds toward adipose tissue regeneration;optimized printability,simulations and surface modification.J Tissue Eng.(2020)
- Shanbhag S et al.Cell therapy for orofacial bone regeneration:a systematic review and meta-analysis.J Clin Periodontol.(2019)
- Hassan MN et al.The Bone Regeneration Capacity of 3D-Printed Templates in Calvarial Defect Models:A Systematic Review and Meta-Analysis.Acta Biomaterialia.(2019)
- Ojansivu M et al.Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells.Biofabrication.(2019)
- Rashad A et al.Inflammatory Responses and Host Tissue Reactions to Wood-Based Nanocellulose Scaffolds.Materials Science&Engineering C Mater Biol Appl.(2019)
Project presentation in international conferences and meetings 2019-2021
- 2021,Shuntaro Yamada,Poster presentation, Long-term low-magnitude fluid shear stress directs bone marrow mesenchymal stem cells toward osteogenic lineage,TERMIS 6th World Congress,Netherland
- 2020,Invited speaker,Kamal Mustafa,2020 Award for Best Research Group at Faculty of Medicine,UiB
- 2020,Invited speakers,Kamal Mustafa and Cecilie Gjerde,Olav Thon Nordic Award
- 2020,Invited speaker,Cecilie Gjerde,Quo Vadis Periodontics Symposium(1 000 participants).
- 2020,Invited speaker,Kamal Mustafa,University of Khartoum,Sudan
- 2020,Invited speaker,Cecilie Gjerde,Forsker Grand Prix meeting,Bergen
- 2020,Invited speaker,Kamal Mustafa,IKO and IGS meeting at UiB
- 2019,Invited speaker,University of Helsinki,Finland
- 2019,Speaker,Cecilie Gjerde,International Faculty Form in Mainz,Germany
- 2019,Speaker,Cecilie Gjerde,ITI Section meeting in Bergen.
- 2019,Speaker,Cecilie Gjerde,IADR,Vancouver,Canada.
- 2019,Speaker,Cecilie Gjerde,Research meeting,University of Tromsø.
- 2019,Keynote Speaker for Plenary Session and Opening Ceremony,Kamal Mustafa,Plenary session,European Association for Osseointegration(EAO)Meeting,Lisbon,Portugal.Title:Are stem cells the implants of the future.
- 2019,Invited Speaker,Kamal Mustafa,NYSCO Annual Meeting,Oslo,Norway.Title:From bench to chairside:Stem cells in bone augmentation.
- 2019,Invited Speaker,Kamal Mustafa,BioVaria Meeting,Munich,Germany.Bone from the 3D printer.
- 2019,Invited Speaker,Kamal Mustafa,AMCHA 2019 Meeting,Sri Lanka.Title:Stem cells in bone regeneration,a systematic approach and pilot study in humans.
- 2018,Invited Speaker,Kamal Mustafa,Joint Workshop in Stem Cells and Regenerative Medicine,Tokyo,Japan.Title:MSC and Biomaterial for Bone Regeneration.
- 2019,Cecilie Gjerde et al.Stem cells in bone regeneration,a clinical trial with 3 years follow-up,Tissue Engineering and Regenerative Medicine(TERMIS),Brisbane,Australia,oral presentation,2019.
- 2019,Jannika Paulamäki.Development of Nanostructured Alginate-Gelatin-Based Inks for 3D Bioprinting of Bone Cells.Biofabrication Conference,Columbus OH,USA,Oral Presentation.
- 2019,Cecilie Gjerde,invited speaker.Bergen Stem Cell Consortium meeting,Bergen Norway.Title:stamcelleforskningen.
- 2019,Shuntaro Yamada.Plasma activation of polymeric biomaterial enhanced initial adhesion and seeding efficiency of mesenchymal stromal cells.Bergen Stem Cell Consortium meeting,Bergen Norway.Oral Presentation(prize for best presentation).
- 2019,Cecilie Gjerde,Invited Speaker;Orthopedic department,Int conf Merch,Norway,Cell therapy induced regeneration in severely atrophied mandibular bone.
- 2019,Cecilie Gjerde,Bergen Dental Health Center,Autumn meeting,Bergen,Norway.Invited Speaker,Beinoppbygging med egne stamceller Bergensmodellen.
- 2019,Cecilie Gjerde,oral presentation,stem cells for bone regeneration,the future is now!IADR,Vancouver,Canada.
- 2019,June.Stem cell research for x-ray,HUS,Norway.Invited Speaker:Cecilie Gjerde.Title:Beinoppbygging med egne stamceller Bergensmodellen
- 2019,Jannika Paulamäki,Characterization of the Printability of Alginate-Gelatin Bioink Blended with Nanocellulose,Scandinavian Society for Biomaterials,Conference,Helsinki,Oral presentation,Award.
